For our Teflon tape Flow injection NH4
analyzer after Hall
& Aller we use either an Amber Science 1056
conductivity meter with a 529 flow-through cell or an
Amber Science 3082 conductivity meter with a 829
flow-through cell.
Unfortunately, these wonderful little instruments have
been discontinued.
As it seems to be hard to find information, we post
everything we know about them here:
Amber Science 1056 conductivity meter manual (pdf)
Amber Science 3082 conductivity meter manual (pdf)
Amber Science 529 and 829 flow-through-cell drawing (pdf)
Amber Science 829 flow-through-cell plug wiring (pdf)
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Opening the conductivity cell housing
Caution: Do not turn the four large hex
screws on the side (5/32" / 4mm Allen). They hold the
measuring cell together. If you untighten those, the cell
starts leaking and ultimately falls apart! You can fix
that, but you will have to take off the case first (which
was what you originally wanted to do).
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829 cell from outside: Four small Allen screws
(1/16" 1.6mm) fix the cell in the cylindrical case. You
have to peel off stickers to see them. The Phillips screws
are used to fix the whole cell in a box. Take them off (!)
before sliding cell out.
Mark the position of the cell in the cylinder case. The
screws that fix the 829 conductivity cell in the
cylindrical housing are four small (1/16" = 1.6mm) grub
screws that can be accessed through holes in the cylinder.
These screw holes are covered by stickers that you have to
peel off.
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Opened conductivity cell: Left:
829 cell. You can see the grub screws turned into the
aluminum plate (1/16 "= 1.6mm Allen). To separate the cell
from the cylinder, turn them clockwise! into the body!
Before sliding out the cell, unscrew the Phillips screw(s)
(on the outside of the cylinder), if there are any,
otherwise it will be hard to slide the cell out of the
cylinder.
The white disk is already disassembled
Right: detailed view of the face of the capillary
which is glued into a black plastic disc.
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Conductivity cell inside:
The flow-through conductivity cell from Amber Scientific
basically consists of two 1/16" stainless steel
capillaries, each with one (529 cell) or two ( 829 cell)
connecting wires and a temperature sensor that is glued
into the capillary holder.
A white plastic (Delrin?) disk is clamped between the two
capillaries and represents the measuring cell length.
The plastic disk is 8mm (5/16 ") thick, the bore is 1 mm
(0.04"),so the opening has a volume of 6.6 µL. The
total length of both capillaries and the bore is 5.5" or
140 mm so the total volume of the amber cell with
connection capillaries is about 116 µL.
The connection capillaries are straight standard HPLC
capillaries with OD 1/16 "(= 1.6mm), ID 0.04" (= 1mm) and
they are 66mm (2.6") long.
The inner sides of the capillaries on both sides of the
white disk represent the electrode surfaces. According to
the manufacturer the cell constant is 100, i.e. only the
last 0.25 mm (1/10") on each side effectively act as
electrode surface.
This corresponds to an area of 0.8 mm2 (a
factor of 0.008 to the standard area of 100 mm2 with cell
constant 1). The cell length of 8 mm corresponds to 0.8
times the nominal distance of the standard cell (10 mm).
At 0.25 mm of the inner capillary wall length effectively
used as an electrode the cell constant is 0.8 / 0.008 =
100.
The type 529 cell has a 6kOhm @ 25°C NTC as
temperature sensor and a 5-pin 180° DIN plug.
The type 829 cell has a double 6kOhm / 30kOhm @ 25 °C
NTC as temperature sensor and an 8-pin 270 ° DIN plug
(with central pin).
This means that the 829 cell may be connected to an old
Amber 1056 measuring device via an adapter (wiring see
below - you cannot buy this adapter). The other way (type
529 cell connected to 3082 meter) it doesn't really work,
unless you simulate the double wires by connecting the two
pins in the 8-pin connector. The second NTC could be
soldered directly into the connector - that is not quite
the temp on the capillary, but it might work for our
purposes. Otherwise you would have to convert the 529 into
an 829 which means changing the plug and the cable,
connecting it with 2 cables per capillary and replacing
the 6kOhm NTC with a 6k/30k double NTC or adding a 30kOhm
NTC.
The conductivity wire connections are made using soldered
connections that are glued into the Delrin capillary
holder with the capillaries. Two soldering tails go to the
capillaries, but the pins are only intended as soldering
points for the thermistor cables which are not (!)
connected to the soldering tails.
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Opened cells: white plastic disc removed.
Left: 829 cell. Right: 529 cell from
2005 (photo credit Sung-Uk An)
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Re-Assembly of the 529 or 829 flow-through conductivity
cell
Carefully center the white plastic disc between the
capillaries and tighten all four large Allen screws (5/32"
4mm) just enough that the disc remains a bit loose. Since
at least three of the screws have Loctite, you have little
feeling for how tight the screws actually are!
One after the other - or rather crosswise - tighten all
four screws just enough to hold the white disk, and then
turn them all back a bit so that the white disk is
loose again.
Use a slightly less than 1mm (0.04") wire to align the
white disk with the capillaries. A thick (= 0.9mm) nylon
wire (fishing line?) or a 0.036" guitar string (that
should be the fifth or A string for an electric guitar) or
maybe a 1/32" (=0.8mm) PEEK capillary will work fine as
well. You might also use the L-shaped cleaning wire that
comes with the cell.
Simply stick the wire gently through the capillary, the
disk and the second capillary. This only works, if the
disk is loose enough. Any bit of misalignment will stop
the wire. Then tighten all four screws a little crosswise
again (approx. 1/4 turn).
Now everything should be straight and tight. (don't
fasten screws too tight - it has to be very straight and
aligned, not very tight !!!).
If you just turn the big Allen screws by feeling, it will
almost certainly be bending the connection a bit and the
cell will be leaking.
Check for leaks: (preferably before refitting the
cylinder case!!)
Place the cell on kitchen paper or paper towels so you see
it leaking. Fill two disposable syringes half way with
water and attach one to each end of the two capillaries
with a short tube (should seal well). E.g. the tube end
with a female luer-lock
connector from a used rhizon
works very well).
If all is fine, you should be able to push the water from
one syringe into the other and, if necessary, apply
pressure to both syringes. if not, it leaks immediately
(hence the cell on kitchen paper.).
If it leaks: Unfasten the screws and carefully try
to reassemble so the white disk sits perfectly flat and
centered between the black pieces.
When everything is "leakproof", insert the cell back into
the cylinder and carefully unscrew (= turn them
counterclockwise!) the grub screws crosswise (!) (1/16
"Allen key) so that the cell unit is centered the
cylinder. The grub screws press against the cylinder from
the inside so to fix the cell they have to be turned
counterclockwise.
As you see, there is not much that could break in those
cells. As long as the capillaries are not blocked and the
inner wall of the capillary does not have stains, the
cells should work properly. Cleaning may be done with the
L-shaped wire provided with the cell or the same wire tha
you use for centering the white disk.
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Amber Science 3082 Conductivity Meter: Serial port
(RS232)
General remark: Only the 3082 model has a digital
output. The serial port only delivers exactly what you see
on the display. For the setup as a detector in a
flow-injection NH4 or CO2 detector
it is more precise to use the analogue signal because you
have a high background conductivity that prevents the
display from showing enough decimals.
Digital recording of data:
Required cable: serial extension (male - female SubD
9-pin)
When you send CR or LF CR to the meter
through the serial connection (or automatically if in
auto-transmit mode) the device outputs data with the
following syntax: (CR = carriage return - hex0D, LF
= line feed - hex 0A)
computer: CRLF
Answer from meter:
Data point xxxLF data point number.
Conductivity xx.xx uSLF 5
digits, could also be x.xxx or xxx.x. µS or mS
Temperature xxx.x Deg CLFCR 2
leading spaces
Auto transmit function
When On: Values are automatically transmitted if
something changes (at least 2 digits) or if you push the
right dial. Settings are transmitted when the left switch
is in "self test" and the right dial is pushed. Parameters
(cell const etc.) cannot be changed
When Off: Parameters may be changed. Output of
data when CR (= hex 0D) is sent from computer
program to RS232
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Left: startup output from 3082 serial port. Right:
data point output after each CR sent to the
serial port
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The Amber scientific software for the 3082
There is a DOS / XP software that comes with the device
that can write data to files
Very basic, works on DOS and Windows XP
Alt-R: write to file (new file or append if file
exists)
Alt-F: format .. as a standard 3 lines are output
to output all info in one line (easier to read) press
Alt-F 1 (not the function key F1!)
Alt-Q: End file (not quit program like it is standard now)
Esc: Quit = exit program completely. The file
is also closed.
We did not check, but the program should also write
directly to a serial printer.
But as mentioned above: Only the digits that can be seen
on the display are transmitted digitally. Thus the
analogue output is actually much more accurate! The major
problem that we have when we use it as a detector in a NH4
flow-injection system is that the background
conductivities are high. We use old Knauer strip chart
recorders that can compensate for quite a bit (but not
all) of zero offset.
A better way would be to directly compensate for the
background conductivity by modifying the setup to become a
Wheaton bridge (parallel to measuring cell R1, a second
cell R2 with pure eluent). With a fixed resistor R3 and an
adjustable resistor R4 you should be able to adjust R1 /
R2 = R3 / R4. So if there is pure eluent in both
conductivity cells, the current should be 0 if the
resistances R3 and R4 are identical
There are precise HPLC conductivity meters that do exactly
this - but they are more expensive than the Amber meters
were.
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Wiring for adapters:
Adapter : 829 cell (270° DIN with central pin) ->
1056 meter (5 pin 180° DIN)
1 and 6 -> 1 electrode 1
3 and 7 -> 3 electrode 2
8 -> 2 ground
5 -> 5 6k NTC
2 -> 4 NTC common
4 not connected (30k NTC)
Adapter : 529 cell - > 3082 meter (8 pin 270°
DIN) this is not precise, but might be working
1 -> 1
electrode 1 cable 1
1
-> 6 electrode 1 cable 2
2
-> 8 ground
3
-> 3 electrode 2 cable 1
3
-> 7 electrode 2 cable 2
4
-> 2 NTC ground
5
-> 5 6k NTC
NTC 30k gnd -> 2
NTC 30k -> 4
Adapter 829 -> WTW LF 191
(7pin 6x60° DIN w central pin)
4 -> 1 NTC 30k
(829=red)
5 -> 2 NTC 6K
(829=green)
2 -> 3 NTC common
(829=yellow)
1 -> 4 electrode 1 cable
1 (829=brown)
6 -> 5 electrode 1 cable
2 (829=blue)
3 -> 6 electrode 2 cable
1 (829=orange)
7 -> 7 electrode 2 cable
2 (829=violet)
8 -> shield (829=blank /
Adapter: black)
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time stamp 03Feb21
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